Effect of Phytic Acid Addition on the Structure of Collagen-Hyaluronic Acid Composite Gel
Author:
Nashchekina Yuliya1ORCID, Guryanov Evgeny1, Lihachev Alexey2, Vaganov Gleb3ORCID, Popova Elena3, Mikhailova Natalya1ORCID, Nashchekin Alexey2ORCID
Affiliation:
1. Center of Cell Technologies, Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky Pr. 4, 194064 St. Petersburg, Russia 2. Laboratory «Characterization of Materials and Structures of Solid State Electronics», Ioffe Institute, Polytekhnicheskaya St. 26, 194021 St. Petersburg, Russia 3. Institute of Macromolecular Compounds of Russian Academy of Sciences, V.O., Bol’shoy Pr. 31, 199004 St. Petersburg, Russia
Abstract
Composite collagen gels with hyaluronic acid are developed tissue-engineered structures for filling and regeneration of defects in various organs and tissues. For the first time, phytic acid was used to increase the stability and improve the mechanical properties of collagen gels with hyaluronic acid. Phytic acid is a promising cross-linker for collagen hydrogels and is a plant-derived antioxidant found in rich sources of beans, grains, and oilseeds. Phytic acid has several benefits due to its antioxidant, anticancer, and antitumor properties. In this work, studies were carried out on the kinetics of the self-assembly of collagen molecules in the presence of phytic and hyaluronic acids. It was shown that both of these acids do not lead to collagen self-assembly. Scanning electron microscopy showed that in the presence of phytic and hyaluronic acids, the collagen fibrils had a native structure, and the FTIR method confirmed the chemical cross-links between the collagen fibrils. DSC and rheological studies demonstrated that adding the phytic acid improved the stability and modulus of elasticity of the collagen gel. The presence of hyaluronic acid in the collagen gel slightly reduced the effect of phytic acid. The presence of phytic acid in the collagen gel improved the stability of the scaffold, but, after 1 week of cultivation, slightly reduced the viability of mesenchymal stromal cells cultured in the gel. The collagen type I gel with hyaluronic and phytic acids can be used to replace tissue defects, especially after the removal of cancerous tumors.
Funder
Russian Science Foundation
Subject
Polymers and Plastics,Organic Chemistry,Biomaterials,Bioengineering
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