N Absorption, Transport, and Recycling in Nodulated Soybean Plants by Split-Root Experiment Using 15N-Labeled Nitrate

Abstract

Nitrate concentration is variable in soils, so the absorbed N from roots in a high-nitrate site is recycled from shoots to the root parts in N-poor niche. In this report, the absorption, transport, and recycling of N derived from 15N-labeled nitrate were investigated with split-root systems of nodulated soybean. The NO3− accumulated in the root in 5 mM NO3− solution; however, it was not detected in the roots and nodules in an N-free pot, indicating that NO3− itself is not recycled from leaves to underground parts. The total amount of 15NO3− absorption from 2 to 4 days of the plant with the N-free opposite half-root accelerated by 40% compared with both half-roots that received NO3−. This result might be due to the compensation for the N demand under one half-root could absorb NO3−. About 2–3% of the absorbed 15N was recycled to the opposite half-root, irrespective of N-free or NO3− solution, suggesting that N recycling from leaves to the roots was not affected by the presence or absence of NO3−. Concentrations of asparagine increased in the half-roots supplied with NO3− but not in N-free half-roots, suggesting that asparagine may not be a systemic signal for N status.