Cytokinin Biosynthesis Is Affected by Selenium and Nitrate Availabilities to Regulate Shoot and Root Growth in Rice Seedlings

Abstract

Selenium (Se) and nitrate have the potential to modify rice root architecture, but it is unclear how Se is linked to changes in the rice seedlings nitrate status. Thus, rice seedlings were grown in nutrient solutions containing either 0- or 10-µM Se that were supplemented with 0.05 (low nitrate condition) or 5.0 mM nitrate (high nitrate condition). Se application to seedlings treated with low nitrate led to sugar accumulation in shoot and root and increased cytokinin concentrations in root, while decreasing cytokinin concentrations in shoot compared with seedlings in 0.05 mM nitrate alone. This, in turn, resulted in decreased shoot growth, while downregulation of OsXTH and OsEXP negatively affected root expansion. On the other hand, Se combined with 5.0 mM nitrate did not affect sugar concentration in tissues compared with seedlings in 5.0 mM nitrate. Moreover, Se negatively regulated the cytokinin biosynthesis in shoot and root of seedlings grown under 5.0 mM nitrate. The reduction in cytokinin concentrations by Se under high nitrate condition decreased shoot growth, but increased root growth through induction of OsXTH and OsEXP. Thus, many of the effects of Se in shoot and root growth are due to a shift in nitrate status of the seedlings.