Ultrasound-Assisted Extraction of Applewood Polyphenols at Lab and Pilot Scales

Author:

Withouck Hannes12ORCID,Paelinck Axel1,Foubert Imogen34ORCID,Fraeye Ilse24ORCID

Affiliation:

1. Biochemical Innovation Team Odisee (BIT-O), Department Chemistry, University College Odisee, Gebroeders De Smetstraat 1, 9000 Ghent, Belgium

2. Meat Technology & Science of Protein-rich Foods (MTSP), Department M2S, Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven—Ghent, Gebroeders De Smetstraat 1, 9000 Ghent, Belgium

3. Research Unit Food & Lipids, KU Leuven Kulak, E. Sabbelaan 53, 8500 Kortrijk, Belgium

4. Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven, Kasteelpark Arenberg 20, 3001 Leuven, Belgium

Abstract

This study focused on the extraction of polyphenols from applewood using ultrasonic-assisted extraction (UAE). First, the influence of solvent composition and mass–volume (m:v) ratio on the extraction yield was studied at a lab scale (200 mL). Overall, a ratio of 1:33 (m:v) resulted in a higher yield of polyphenols. Furthermore, both a higher yield of polyphenols and higher antioxidant capacity were detected in the extracts produced in the presence of a 30 v/v % ethanol mixture compared to pure water; a further increase in ethanol did not improve the extraction yield. Second, under the optimal conditions (30 v/v % ethanol–water; 1:33 and 1:20 (m:v)), the UAE technique was applied at a pilot scale (100 L). At 1:33 (m:v), the polyphenol yield was lower at the pilot scale compared to the lab scale; by contrast, at 1:20 (m:v), production at the pilot scale resulted in a higher yield compared to the lab scale. To identify and quantify individual polyphenols, HPLC-PDA analyses were performed. Phloridzin appears to be the major identified compound. Finally, the UAE process was compared to a conventional solid–liquid extraction technique, showing that a significantly higher yield could be obtained with UAE.

Funder

Flemish Government

Publisher

MDPI AG

Subject

Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science

Reference61 articles.

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