Chemical and Bioactive Screening of Green Polyphenol-Rich Extracts from Chestnut By-Products: An Approach to Guide the Sustainable Production of High-Added Value Ingredients
Author:
Rodrigues Daniele Bobrowski12, Veríssimo Lavínia12ORCID, Finimundy Tiane12ORCID, Rodrigues Joana12ORCID, Oliveira Izamara12, Gonçalves João3, Fernandes Isabel P.3ORCID, Barros Lillian12ORCID, Heleno Sandrina A.12ORCID, Calhelha Ricardo C.12ORCID
Affiliation:
1. Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal 2. Laboratório Associado para a Sustentabilidade e Tecnologia em Regiões de Montanha (SusTEC), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal 3. Tree Flowers Solutions, Lda, Edificio Brigantia Ecopark, Av. Cidade de Léon, 5300-358 Bragança, Portugal
Abstract
Opportunities for the valorisation of agro-industrial residues of the chestnut (Castanea sativa Mill.) production chain have been fostered with the production of multifunctional polyphenol-rich extracts with the potential to be introduced as natural additives or active components in several products. Nonetheless, it is crucial to explore the feasibility of different extracts from the various by-products for these applications through the exhaustive study of their composition and bioactivities without losing sight of the sustainable character of the process. This work aimed at the screening of the phenolic compound composition and bioactivities of different green extracts of chestnut burs, shells and leaves, as the first step to establish their potential application as natural ingredients, primarily as food preservatives. To this end, maceration (MAC) as a conventional extraction method besides ultrasound and microwave-assisted extractions (UAE and MAE) was employed to obtain the extracts from chestnut by-products using water (W) and hydroethanolic solution (HE) as solvents. Phenolic compounds were analysed by HPLC-DAD-(ESI-)MS/MS; the antioxidant capacity was assessed by colourimetric assays, and the antimicrobial activity was evaluated against several strains of food-borne bacteria and fungi. The leaf extracts obtained by MAC-HE and UAE-HE presented the highest concentration of phenolic compounds (70.92 ± 2.72 and 53.97 ± 2.41 mg.g−1 extract dw, respectively), whereas, for burs and shells, the highest recovery of total phenolic compounds was achieved by using UAE-HE and UAE-W (36.87 ± 1.09 and 23.03 ± 0.26 mg.g−1 extract dw, respectively). Bis-HHDP-glucose isomers, chestanin and gallic acid were among the most abundant compounds. Bur extracts (MAC-HE and UAE-HE) generally presented the highest antioxidant capacity as measured by TBARS, while the best results in DPPH and reducing power assays were found for shell extracts (MAE-W and MAC-HE). Promising antibacterial activity was noticed for the aqueous extracts of burs, leaves and hydroethanolic extracts of shells, with emphasis on the MAE-W extract of burs that showed bactericidal activity against E. cloacae, P. aeruginosa and S. aureus (MBC 5 mg.mL−1). Overall, it can be concluded that chestnut by-products, including burs, shells and leaves, are sources of polyphenolic compounds with significant antioxidant and antimicrobial activities. The choice of extraction method and solvent greatly influenced the composition and bioactivity of the extracts. These findings highlight the potential of chestnut by-products for the development of natural additives, particularly for food preservation, while also emphasizing the importance of sustainable utilization of agricultural waste materials. Further research is warranted to optimize extraction techniques and explore additional applications for these valuable bioactive compounds.
Funder
Foundation for Science and Technology European Regional Development Fund
Subject
Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science
Reference68 articles.
1. Food and Agriculture Organization (2023, April 07). Technical Platform on the Measurement and Reduction of Food Loss and Waste. Available online: https://www.fao.org/platform-food-loss-waste/. 2. Green food analysis: Current trends and perspectives;Mendiola;Curr. Opin. Green Sustain. Chem.,2021 3. Making sense of the “clean label” trends: A review of consumer food choice behavior and discussion of industry implications;Asioli;Food Res. Int.,2017 4. Natural and healthy? Consumers knowledge, understanding and preferences regarding naturalness and healthiness of processed foods;Saulais;Int. J. Gastron. Food Sci.,2023 5. Witkowski, M., Grajeta, H., and Gomułka, K. (2022). Hypersensitivity reactions to food additives-preservatives, antioxidants, flavor enhancers. Int. J. Environ. Res. Public Health, 19.
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