Microbiological Characterization of Protected Designation of Origin Serra da Estrela Cheese

Author:

Rocha Rui1ORCID,Couto Nélson2,Pinto Ricardo Pereira1ORCID,Vaz-Velho Manuela12ORCID,Fernandes Paulo12ORCID,Santos Joana12ORCID

Affiliation:

1. CISAS—Center for Research and Development in Agrifood Systems and Sustainability, Instituto Politécnico de Viana do Castelo, Rua Escola Industrial e Comercial de Nun’Álvares, 4900-347 Viana do Castelo, Portugal

2. Escola Superior de Tecnologia e Gestão, Instituto Politécnico de Viana do Castelo, Avenida do Atlântico no. 644, 4900-348 Viana do Castelo, Portugal

Abstract

Serra da Estrela is the oldest and most recognizable traditional protected designation of origin (PDO) cheese from Portugal. It has been extensively studied over the years, but the latest microbial characterization is 20 years old. Hence, this work aimed to perform an updated characterization of Serra da Estrela PDO cheeses and raw materials. Our analysis showed that lactic acid bacteria content on Serra da Estrela cheeses exceeded 8.8 log CFUsg−1, in all analyzed samples, with lactococci, lactobacilli and Leuconostoc spp. predominating over enterococci strains. Moreover, lactococci and lactobacilli abundance increased across the production season, while enterococci dropped considerably in late manufactures. Lastly, Leuconostoc spp. content remained unchanged in all analyzed periods. A correspondence analysis showed that L. paracasei, L. lactis, E. durans, E. faecium and L. mesenteroides are transversal in Serra da Estrela cheese manufacturing and were closely associated with milk, curd and cheese matrices. Additionally, L. casei, L. plantarum and L. curvatus were specifically associated with cheese matrices, possibly active during ripening and contributing for the development of these cheeses’ organoleptic characteristics.

Funder

MobFood—Mobilizing scientific and technological knowledge in response to the challenges of the agri–food market

European Regional Development Fund

FCT–Fundação para a Ciência e a Tecnologia

Publisher

MDPI AG

Subject

Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science

Reference54 articles.

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3. A Review on the General Cheese Processing Technology, Flavor Biochemical Pathways and the Influence of Yeasts in Cheese;Zheng;Front. Microbiol.,2021

4. Papademas, P., and Bintsis, T. (2017). Global Cheesemaking Technology: Cheese Quality and Characteristics, John Wiley & Sons Ltd.. [1st ed.].

5. Cotter, P.D., and Everett, D.W. (2017). Cheese−Chemistry, Physics and Microbiology, Academic Press. [4th ed.].

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