Optimization of Water Lentil (Duckweed) Leaf Protein Purification: Identification, Structure, and Foaming Properties
Author:
Muller Tristan12, Bernier Marie-Ève12, Bazinet Laurent12ORCID
Affiliation:
1. Institute of Nutrition and Functional Foods (INAF), Department of Food Sciences, Université Laval, Quebec, QC G1V 0A6, Canada 2. Laboratoire de Transformation Alimentaire et Procédés Électro Membranaires (LTAPEM), Laboratory of Food Processing and Electro Membrane Processes, Université Laval, Quebec, QC G1V 0A6, Canada
Abstract
Water lentil, commonly known as duckweed, is an aquatic plant with great agronomic potential, as it can double its biomass in less than 24 h and contains up to 45% leaf proteins on a dry matter basis. However, extracting proteins from leaves is an arduous process due to the complexity of the matrix, which limits their uses in the food industry. In this study, water lentil protein extraction by solubilization was maximized using response surface methodology. By heating at 80 °C at pH 11 with a water lentil powder concentration of 2% or 4% for 2 h, up to 77.8% of total proteins were solubilized. Then, by precipitating the solubilized proteins at pH 4, a protein purity of 57.6% combined with a total protein yield of 60.0% was achieved. To the best of our knowledge, this is the highest leaf protein extraction yield reported in the literature with such protein purity. Proteomics analyses showed that the protein concentrate was composed of around 85.0% RubisCO, and protein structure analyses using ATR-FTIR and DSC were linked to a high protein solubility in water at pH 7. Moreover, a 1.5% protein solution of the protein concentrate at pH 7 showed excellent foaming properties compared to a 10.3% protein egg white solution. It had a superior foaming capacity (194% vs. 122%, respectively) for the same foaming stability after 60 min, which confirms water lentil proteins’ potential for human nutrition and food formulation.
Funder
Consortium de Recherche et Innovations en Bioprocédés Industriels au Québec Natural Sciences and Engineering Research Council of Canada
Subject
Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science
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