A High-Performance Liquid Chromatography with Electrochemical Detection Method Developed for the Sensitive Determination of Ascorbic Acid: Validation, Application, and Comparison with Titration, Spectrophotometric, and High-Performance Liquid Chromatography with Diode-Array Detection Methods
Author:
Wu Fanhua1, Xu Fangrui1, Liu Wen1, Chen Sinan1, Luo Haojie1, Cheng Ni12, Zhao Haoan12ORCID, Cao Wei12
Affiliation:
1. College of Food Science and Technology, Northwest University, Xi’an 710069, China 2. Bee Product Research Center of Shaanxi Province, Xi’an 710065, China
Abstract
L-ascorbic acid (vitamin C, VC), an essential nutrient obtained from the diet to maintain various vital signs for the human body, is a crucial indicator of food quality and nutritional value. Herein, high-performance liquid chromatography with electrochemical detection (HPLC-ECD) was developed and validated with the advantages of higher sensitivity, simpler operation processes, and more rapid detection in measuring VC levels in honey samples when compared with the common methods (titration, spectrophotometric, and HPLC-DAD methods). The results of the HPLC-ECD methodological validation showed that the limit of detection (LOD) was 0.0043 µg mL−1; the relative standard deviations (RSDs) of the intra- and inter-day values were between 2.51% and 5.15%, and the regression coefficient was >0.999 in the linear range of 0.1 to 20 µg mL−1. The validated HPLC-ECD method was also successfully utilized to evaluate the VC levels in different varieties of honey samples with various storage durations as well as in fruit and biological samples. This study provided a perspective for the further accurate determination of VC content in food and biological samples.
Funder
National Key R&D Project Xi’an Science and Technology Planning Project Shaanxi High-Level Talent Special Support Plan
Subject
Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science
Reference38 articles.
1. Rowe, S., and Carr, A.C. (2020). Global vitamin C status and prevalence of deficiency: A cause for concern?. Nutrients, 12. 2. Targeting cancer vulnerabilities with high-dose vitamin C;Ngo;Nat. Rev. Cancer,2019 3. Kashiouris, M.G., L’Heureux, M., Cable, C.A., Fisher, B.J., Leichtle, S.W., and Fowler, A.A. (2020). The emerging role of vitamin C as a treatment for sepsis. Nutrients, 12. 4. Paciolla, C., Fortunato, S., Dipierro, N., Paradiso, A., De Leonardis, S., Mastropasqua, L., and de Pinto, M.C. (2019). Vitamin C in plants: From functions to biofortification. Antioxidants, 8. 5. Vitamin C content in fruits: Biosynthesis and regulation;Fenech;Front. Plant Sci.,2019
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|