Effects of Nitrogen and Phosphorus Additions on Soil N2O Emissions and CH4 Uptake in a Phosphorus-Limited Subtropical Chinese Fir Plantation

Abstract

Increased nitrogen (N) inputs in subtropical forest ecosystems were widely reported. Extra N additions were reported to cause nutrient imbalance and phosphorus (P) limitation in many tropical and subtropical forests, and further result in changes in soil nitrous oxide (N2O) and methane (CH4) fluxes. Here, we conducted experiments with N (high N addition: 15 g N/m2, HN), P (low: 5 g P/m2, LP; high: 15 g P/m2, HP) and their interactive (HNLP and HNHP) treatments to investigate how N and P additions affected CH4 and N2O exchanges in an N-rich Chinese fir plantation (Cunninghamia lanceolata), and further explored the underlying mechanisms through the structural equation model (SEM) analysis. The results indicated that N addition alone (HN) significantly (p < 0.05) increased the soil N2O emissions by 30.15% and 80.47% over annual and 4-month periods, mainly owing to the elevated NH4+-N content. P addition alone (LP and HP) did not significantly affect the soil N2O emissions as compared with the control. The SEM analysis indicated that increased N2O emissions under N addition were primarily explained by the increase in available N and contributed more to the stimulated NH4+-N contents. N and P interactive additions slightly (not significant) stimulated the N2O emissions as compared with that under the N addition alone treatment. High-dose P addition significantly increased the soil CH4 uptake by 15.80% and 16.23% under the HP and HNHP treatments, respectively, while N addition alone and low P addition (LP and HNLP) did not significantly affect CH4 uptake as compared with the control. The increased water-soluble organic carbon and microbial biomass carbon explained the increased CH4 uptake under high P addition. The fertilization effects on N2O emissions and CH4 uptake mainly occurred in the first 4 months and diminished after that. Our results suggested that the direction, magnitude and timing of the N and P addition effects on N2O emissions and CH4 uptake would depend on the soil nutrient status and plant–microbial competition for N and P in subtropical forests.