Affiliation:
1. Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China
Abstract
Alfalfa (Medicago sativa) is an important leguminous forage, known as the “The Queen of Forages”. Abiotic stress seriously limits the growth and development of alfalfa, and improving the yield and quality has become an important research area. However, little is known about the Msr (methionine sulfoxide reductase) gene family in alfalfa. In this study, 15 Msr genes were identified through examining the genome of the alfalfa “Xinjiang DaYe”. The MsMsr genes differ in gene structure and conserved protein motifs. Many cis-acting regulatory elements related to the stress response were found in the promoter regions of these genes. In addition, a transcriptional analysis and qRT-PCR (quantitative reverse transcription PCR) showed that MsMsr genes show expression changes in response to abiotic stress in various tissues. Overall, our results suggest that MsMsr genes play an important role in the response to abiotic stress for alfalfa.
Funder
National Natural Science Foundation of China
First-class Grassland Science Discipline Program of Shandong Province
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference57 articles.
1. Alfalfa-Most Important Perennial Forage Legume in Animal Husbandry;Biotechnol. Anim. Husb.,2009
2. Acharya, J.P., Lopez, Y., Gouveia, B.T., de Bem Oliveira, I., Resende, M.F.R., Muñoz, P.R., and Rios, E.F. (2020). Breeding Alfalfa (Medicago sativa L.) Adapted to Subtropical Agroecosystems. Agronomy, 10.
3. Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa;Chen;Nat. Commun.,2020
4. The formation, resolution, and optical properties of the diastereoisomeric sulfoxides derived from L-methionine;Lavine;J. Biol. Chem.,1947
5. The oxidative environment and protein damage;Davies;Biochim. Biophys. Acta,2005