Toxic Effect of Ammonium Nitrogen on the Nitrification Process and Acclimatisation of Nitrifying Bacteria to High Concentrations of NH4-N in Wastewater

Abstract

The aim of the conducted research was to assess the effectiveness of the nitrification process, at different concentrations of ammonium nitrogen, in biologically treated wastewater in one of the largest municipal and industrial wastewater treatment plants in Poland. The studies also attempted to acclimate nitrifying bacteria to the limited concentration of ammonium nitrogen and determined the efficiency of nitrification under the influence of acclimated activated sludge in the biological wastewater treatment system. The obtained results indicate that the concentration of ammonium nitrogen above 60.00 mg·dm−3 inhibits nitrification, even after increasing the biomass of nitrifiers. The increase in the efficiency of the nitrification process in the tested system can be obtained by using the activated sludge inoculated with nitrifiers. For this purpose, nitrifiers should be preacclimated, at least for a period of time, allowing them to colonize the activated sludge. The acclimated activated sludge allows reducing the amount of ammonium nitrogen in treated sewage by approx. 35.0%. The process of stable nitrification in the biological treatment system was observed nine days after introducing the acclimated activated sludge into the aeration chamber.