Comparative Proteomics of the Acanthopagrus schlegelii Gonad in Different Sex Reversal

Abstract

A substantial proportion of Acanthopagrus schlegelii individuals change sex from male to female during their lifetime. However, the mechanisms underlying sex change are unknown. In this research, iTRAQ analyses of proteins obtained from A.schlegelii gonads in four different stages of development were compared. In total, 4692 proteins were identified, including common sex-specific proteins, such as sperm-associated antigen 6 and cilia- and flagella-associated proteins in males, and zona pellucida sperm-binding proteins in females. Furthermore, proteins involved in the integrin signaling pathway, inflammation mediated by the chemokine and cytokine signaling pathways, pyruvate metabolism, CCKR signaling map, de novo purine biosynthesis and the ubiquitin proteasome pathway were upregulated in female gonads, whereas proteins implicated in DNA replication, the heterotrimeric G-protein signaling pathway, Gi alpha- and Gs alpha-mediated pathways, wnt signaling pathway, and hedgehog signaling pathway were upregulated in male gonads. Interestingly, cathepsins were only identified in ovaries, indicating their potential involvement in rapid ovarian development. Apoptosis-related proteins expressed in ovaries (such as MAPK and Cdc42) may protect them from cancer. This is the first report on the gonad proteome from A.schlegelii in different stages of sex reversal, and the results provide important fundamental data for studying the molecular mechanisms of sex reversal.