Imaging Differential Mercury Species Bioaccumulation in Glass Eels Using Isotopic Tracers and Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Abstract

Dramatic increases in global mercury pollution require a deeper understanding of specific toxicity mechanisms for mercury compounds in organisms. Despite numerous studies addressing mercury toxicity, the detailed mechanisms underlying its transport and accumulation in fish remain unclear. The aim of this study was to unravel differential uptake pathways for mercury compounds, metabolisation, and sequestration mechanisms in glass eels using techniques able to localize at the tissue and organ levels. A multi isotope image mapping procedure was developed to simultaneously study the uptake and distribution of both mercury compounds MeHg and Hg(II) within the organs of the whole organism. The use of isotopically labelled Hg species (methylmercury Me201Hg and inorganic mercury 199Hg(II)) and image based on isotope ratio instead of elemental signals allowed to visualize spatially and with time the differential Hg species uptake, transport, and sequestration routes. The results showed a preferential uptake of the MeHg counterpart and a dynamic transport of MeHg within different organs. The gills were the main target organs for MeHg uptake, whereas the skeletal muscle was the final MeHg storage tissue. Hg(II) was found to mainly transit by the gills and the olfactory bulbs with a very low transfer and storage in the other organs and a rapid depuration. No significant internal demethylation and methylation was observed during this experimentation.