Continuous Real-Time Detection of Serotonin Using an Aptamer-Based Electrochemical Biosensor

Author:

Ahmad Habib M. N.1,Andrade Arturo23,Song Edward1ORCID

Affiliation:

1. Department of Electrical & Computer Engineering, University of New Hampshire, Durham, NH 03824, USA

2. Robert J. & Nancy D. Carney Institute for Brain Science, Brown University, Providence, RI 02912, USA

3. Department of Neuroscience, Brown University, Providence, RI 02912, USA

Abstract

Serotonin (5-HT) is a critical neurotransmitter involved in many neuronal functions, and 5-HT depletion has been linked to several mental diseases. The fast release and clearance of serotonin in the extracellular space, low analyte concentrations, and a multitude of interfering species make the detection of serotonin challenging. This work presents an electrochemical aptamer-based biosensing platform that can monitor 5-HT continuously with high sensitivity and selectivity. Our electrochemical sensor showed a response time of approximately 1 min to a step change in the serotonin concentration in continuous monitoring using a single-frequency EIS (electrochemical impedance spectroscopy) technique. The developed sensing platform was able to detect 5-HT in the range of 25–150 nM in the continuous sample fluid flow with a detection limit (LOD) of 5.6 nM. The electrochemical sensor showed promising selectivity against other species with similar chemical structures and redox potentials, including dopamine (DA), norepinephrine (NE), L-tryptophan (L-TP), 5-hydroxyindoleacetic acid (5-HIAA), and 5-hydroxytryptophan (5-HTP). The proposed sensing platform is able to achieve high selectivity in the nanomolar range continuously in real-time, demonstrating the potential for monitoring serotonin from neurons in organ-on-a-chip or brain-on-a-chip-based platforms.

Funder

National Institute of Biomedical Imaging and Bioengineering

National Science Foundation

National Institute of General Medical Sciences of the National Institutes of Health

National Institute of Mental Health

Publisher

MDPI AG

Subject

Clinical Biochemistry,General Medicine,Analytical Chemistry,Biotechnology,Instrumentation,Biomedical Engineering,Engineering (miscellaneous)

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