Role of Lactiplantibacillus plantarum UBLP-40, Lactobacillus rhamnosus UBLR-58 and Bifidobacterium longum UBBL-64 in the Wound Healing Process of the Excisional Skin
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Published:2023-04-10
Issue:8
Volume:15
Page:1822
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ISSN:2072-6643
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Container-title:Nutrients
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language:en
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Short-container-title:Nutrients
Author:
Panagiotou Dimitrios1, Filidou Eirini23ORCID, Gaitanidou Maria2, Tarapatzi Gesthimani23ORCID, Spathakis Michail23ORCID, Kandilogiannakis Leonidas23ORCID, Stavrou George4, Arvanitidis Konstantinos23ORCID, Tsetis Joulia K.5, Gionga Persefoni4, Shrewsbury Anne D.4, Manolopoulos Vangelis G.23ORCID, Kapoukranidou Dora6, Lasithiotakis Konstantinos1ORCID, Kolios George23ORCID, Kotzampassi Katerina4ORCID
Affiliation:
1. Department of Surgery, School of Medicine, University of Crete, 71003 Heraklion, Greece 2. Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, 68100 Alexandroupolis, Greece 3. Individualised Medicine & Pharmacological Research Solutions Center (IMPReS), 68100 Alexandroupolis, Greece 4. Department of Surgery, Aristotle University of Thessaloniki, 54636 Thessaloniki, Greece 5. Pharmacist MSc, Uni-Pharma S.A., 14564 Athens, Greece 6. Department of Physiology, Faculty of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
Abstract
The probiotics Lactiplantibacillus plantarum UBLP-40, Lactobacillus rhamnosus UBLR-58 and Bifidobacterium longum UBBL-64 seem to promote wound healing when applied topically. Our aim was to investigate their effect on the mRNA expression of pro-inflammatory, healing and angiogenetic factors during the healing process of a standardized excisional wound model in rats. Rats subjected to six dorsal skin wounds were allocated to Control; L. plantarum; combined formula of L. rhamnosus plus B. longum; L. rhamnosus; and B. longum treatments, applied every two days, along with tissue collection. The pro-inflammatory, wound-healing, and angiogenetic factors of mRNA expression were assessed by qRT-PCR. We found that L. plantarum exerts a strong anti-inflammatory effect in relation to L. rhamnosus–B. longum, given alone or in combination; the combined regime of L. rhamnosus–B. longum, works better, greatly promoting the expression of healing and angiogenic factors than L. plantarum. When separately tested, L. rhamnosus was found to work better than B. longum in promoting the expression of healing factors, while B. longum seems stronger than L. rhamnosus in the expression of angiogenic factors. We, therefore, suggest that an ideal probiotic treatment should definitively contain more than one probiotic strain to speed up all three healing phases.
Funder
project IMPReS Program “Competitiveness, Entrepreneurship and Innovation” Greece and the European Union
Subject
Food Science,Nutrition and Dietetics
Reference46 articles.
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