Exogenous Methyl Jasmonate (MeJA) Improves ‘Ruixue’ Apple Fruit Quality by Regulating Cell Wall Metabolism

Author:

Ding Xiaoyi1,Wang Bin1,Gong Yubo1,Yan Xueqing1,Chen Xinxin1,Zhong Yuanwen1,Zhao Zhengyang1

Affiliation:

1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Xianyang 712100, China

Abstract

‘Ruixue’ apples were used as the test material to study the effect of 10 μM methyl jasmonate (MeJA) on the quality and cell wall metabolism of apples after 18 d of storage. The results showed that MeJA significantly decreased the respiratory rate, reduced the titratable acid content and maintained a high soluble solids content. MeJA has been shown to suppress the activities and gene expressions of WSP, CSP, ISP, and cellulose in contrast to the control group, thereby maintaining a lower cell permeability and higher exocarp firmness. MeJA significantly decreased the expression of MdACS, MdACO, MdPL, Mdgal, and MdPG genes in the apple exocarp when compared to the control group. In addition, the overexpression of MdPL18 increased the content of cell wall polysaccharides such as WSP and CSP, enhanced cell wall-degrading enzyme activities, and accelerated fruit ripening and softening, whereas silencing MdPL18 did the opposite. Together, these results demonstrate that exogenous MeJA maintains the Ruixue apple fruit quality by regulating the metabolism of cell wall substances.

Funder

Shaanxi Engineering Research Center of Apple, China

Weinan Experimental Demonstration Station Construction Project of Northwest A&F University

Earmarked Fund for China Agriculture Research System

Publisher

MDPI AG

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