Extraction, Identification, and Antioxidant Activity of Flavonoids from Hylotelephium spectabile (Boreau) H. Ohba
Author:
Li Na12, Wu Xiao13, Yin Qin13, Dong Zeng13ORCID, Zheng Lele1, Qian Yihui1, Sun Yulu1, Chen Ziping2, Zhai Kefeng13ORCID
Affiliation:
1. School of Biological and Food Engineering, Suzhou University, Suzhou 234000, China 2. Anhui Promotion Center for Technology Achievements Transfer, Anhui Academy of Science and Technology, Hefei 230031, China 3. Engineering Research Center for Development and High Value Utilization of Genuine Medicinal Materials in North Anhui Province, Suzhou 234000, China
Abstract
The extraction of total flavonoids from Hylotelephium spectabile (Boreau) H. Ohba (H. spectabile) leaves was studied through the use of a double enzyme-assisted ultrasonic method, and the extraction process was optimized using the Box–Behnken design. Eight different macroporous resins were screened for purification in single-factorial experiments, and the flavonoid compounds in the extract of H. spectabile leaves were identified using HPLC-MS. Through the evaluation of the total reducing capacity and capacity for reducing 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), hydroxyl radicals (·OH), and 2,2’-biazobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), the in vitro antioxidant activities of the crude extracts of the total flavonoids and purified total flavonoids of H. spectabile leaves were investigated. The results showed that the most efficient conditions for flavonoid extraction were an ultrasonic extraction time of 60 min, an ethanol concentration of 35%, a liquid-to-material ratio of 20:1 mL/g, and an amount of enzyme (cellulose/pectinase = 1:1) of 1.5%, forming H. spectabile powder. Under these conditions, the total flavonoid extraction rate in the H. spectabile leaf extract was 4.22%. AB-8 resin showed superior performance in terms of purification, and the optimal adsorption and desorption times were 1.5 h and 3 h, respectively. The recommended parameters for purification included a liquid volume of 5.5 BV, a flow rate of 1.2 BV/min, a pH of 5, and a concentration of 0.8 mg/mL. The observed order for reducing capacity was ascorbic acid (VC) > rutin > purified total flavonoids > crude extract of total flavonoids. The purified total flavonoid extract from H. spectabile showed a good scavenging ability against DPPH, ·OH, and ABTS·+, suggesting strong antioxidant activity. Therefore, this study can serve as technical support and reference data for the further development and utilization of H. spectabile resources.
Funder
Key Research Project of the Anhui Education Department Key research projects of Suzhou University Talent Cultivation Innovation-Biotechnology Anhui Provincial First-class Professional Construction
Reference60 articles.
1. Ecophysiological and Micromorphological Characterization of Green Roof Vegetation for Urban Mitigation;Baraldi;Urban For. Urban Green.,2019 2. Alamgir, A.N.M. (2017). Medicinal, Non-Medicinal, Biopesticides, Color- and Dye-Yielding Plants; Secondary Metabolites and Drug Principles; Significance of Medicinal Plants; Use of Medicinal Plants in the Systems of Traditional and Complementary and Alternative Medicines (CAMs). Therapeutic Use of Medicinal Plants and Their Extracts: Volume 1: Pharmacognosy, Springer International Publishing. 3. Gunathilaka, T.L., Samarakoon, K.W., Ranasinghe, P., and Peiris, L.D.C. (2019). In-Vitro Antioxidant, Hypoglycemic Activity, and Identification of Bioactive Compounds in Phenol-Rich Extract from the Marine Red Algae Gracilaria edulis (Gmelin) Silva. Molecules, 24. 4. Chemical Constituents of Hylotelephium Erythrostictum;Fang;Chem. Nat. Compd.,2021 5. Boriollo, M.F.G., Marques, M.B., da Silva, T.A., da Silva, J.J., Dias, R.A., Silva Filho, T.H.N., Melo, I.L.R., dos Santos Dias, C.T., Bernardo, W.L.d.C., and de Mello Silva Oliveira, N. (2020). Antimicrobial Potential, Phytochemical Profile, Cytotoxic and Genotoxic Screening of Sedum Praealtum A. DC. (Balsam). BMC Complement. Med. Ther., 20.
|
|