The Ethanol Production from Sugar Beet Pulp Supported by Microbial Hydrolysis with Trichoderma viride

Abstract

Despite the significant progress in the research, the problem of finding an efficient method for producing bioethanol from renewable lignocellulosic waste materials remains unresolved. Our investigation aimed to assess the efficacy of ethanol production from sugar beet pulp (SBP) utilising various approaches, including pretreatment variations, enzymatic processes, and microbial hydrolysis. Our research involved using the post-cultivation concentrate of T. viride LOCK 0588 grown in the SBP-based medium as a source of enzymes. The SBP hydrolysis process was carried out for 48 h at 50 °C. The quantity of sugar released, up to 61 g dm−3, through the utilisation of this extract proved to be on par with the outcomes achieved by the application of the commercial Cellic Ctec2 preparation. The final yields of the ethanol production with the use of the coculture of S. cerevisiae (Ethanol Red) and Scheffersomyces stipitis LOCK 0047 strain were in the range 5.1 ± 0.11 kg 100 kg−1 ÷ 5.38 ± 0.11 kg 100 kg−1. These results provide a solid basis for improving larger-scale industrial procedures that involve converting SBP into bioethanol using a cost-efficient approach of microbial hydrolysis with T. viride and a blend of pentose and hexose fermenting yeast.