Identification of Duplication Genotypes of the Feathering Rate Gene in Chicken by a Multiplex PCR Following Electrophoresis and/or Sanger Sequencing

Author:

Shen Qingmiao1,Li Junying1,Bao Haigang1ORCID,Wu Changxin1

Affiliation:

1. National Engineering Laboratory for Animal Breeding, Beijing Key Laboratory of Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

Abstract

Sex-linked phenotypes of late feathering (LF) and early feathering (EF) are controlled by a pair of alleles K and k+. Autosexing based on the feathering rate is widely used in poultry production. It is reported that a tandem duplication of 176,324 base pairs linked to the K locus is responsible for LF expression and could be used as a molecular marker to detect LF chicken. So far, there is no genotyping method that can accurately and stably identify the LF homozygote and heterozygote in all chicken breeds. In the present study, a multiplex PCR test was developed to identify EF, LF homozygote, and heterozygote according to electrophoretic bands and the relative height of the peaks by Sanger sequencing. We tested 413 chickens of six native Chinese breeds with this method. The identification was consistent with the sex and phenotype records of the chickens. Band density analysis was performed, and the results supported our genotyping using the new assay. In order to further verify the accuracy of this test in distinguishing homozygote and heterozygote males, 152 LF males were mated with EF females, and the results of the offspring’s phenotypes were consistent with our expectations. Our results support tandem duplication as molecular markers of LF, and this new test is applicable to all LF chickens associated with tandem duplication.

Funder

National Key Research and Development Program of China

China Agriculture Research System

Publisher

MDPI AG

Subject

General Veterinary,Animal Science and Zoology

Reference33 articles.

1. Changes in plumage color and patterns in Polbar breed chicks (Polish conservative breed) during their first weeks after hatching;Gryzinska;Eur. Poult. Sci.,2014

2. Masui, K., and Hashimoto, J.J.S.B.C. (1933). Sexing Baby Chicks, Journal Printing Co., Limited.

3. Research on Feather Rate and Feather Type of Chicken in Different Strains and Their Offspring;Li;J. Sichuan Agric. Univ.,1988

4. Crossing-over involving three sex-linked genes in chickens;Serebrovsky;Am. Nat.,1922

5. Inheritance of rate of feathering in poultry;Warren;J. Hered.,1925

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