Machine Learning-Based Models for Detection of Biomarkers of Autoimmune Diseases by Fragmentation and Analysis of miRNA Sequences

Abstract

Thanks to high-throughput data technology, microRNA analysis studies have evolved in early disease detection. This work introduces two complete models to detect the biomarkers of two autoimmune diseases, multiple sclerosis and rheumatoid arthritis, via miRNA analysis. Based on work the authors published previously, both introduced models involve complete pipelines of text mining methods, integrated with traditional machine learning methods, and LSTM deep learning. This work also studies the fragmentation of miRNA sequences to reduce the needed processing time and computational power. Moreover, this work studies the impact of obtaining two different library preparation kits (NEBNEXT and NEXTFLEX) on the detection accuracy for rheumatoid arthritis. Additional experiments are applied to the proposed models based on three different transcriptomic datasets. The results denote that the transcriptomic fragmentation model reported a biomarker detection accuracy of 96.45% on a sequence fragment size of 0.2, indicating a significant reduction in execution power while retaining biomarker detection accuracy. On the other hand, the LSTM model obtained a promising detection accuracy of 72%, implying savings in feature engineering processing. Additionally, the fragmentation model and the LSTM model reported 22.4% and 87.5% less execution time than work in the literature, respectively, denoting a considerable execution power reduction.