Fast Determination of Rutin on a Biosensor Made Using a Layered Double Hydroxide Nanocomposite Modified Electrode

Author:

Liu Yuge1,Li Zhiguo1,Chen Weizhen1,Feng Xiaomiao2

Affiliation:

1. Key Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, School of Chemistry and Chemical Engineering, Lingnan Normal University, Zhanjiang 524048, China

2. Key Laboratory for Organic Electronics and Information Displays, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing 210023, China

Abstract

In this study, a nanocomposite of LDH/graphene/polyaniline/gold (LDH/rGO/PANI/Au) was synthesized and characterized. The results of characterization showed that the composite material preserved the layered structure of LDH. The composite was dropped onto the glassy carbon electrode and laccase was then immobilized. Electrochemical tests showed that the composite could accelerate the electron transfer between the enzyme and the electrode. The composite/laccase showed an obvious response to rutin and the optimal detection conditions were discussed. The oxidative peak current of the biosensor constructed using the modified electrode was negatively correlated with rutin in the range of 0.05–4 μg/mL. The detection limit was 0.0017 μg/mL at a signal-to-noise ratio of 3. This biosensor of rutin also possessed high sensitivity, excellent anti-interference ability, and stability. The contents of rutin in tablets, first determined using HPLC, were also detected using the sensor constructed in this research as an application, and the results were acceptable. This research here provides a facile way for the fast detection of rutin in real samples.

Funder

Special Talent Fund of Lingnan Normal University

Guangdong Basic and Applied Basic Research Foundation

Open Research Fund of Key Laboratory of Jiangsu Province

Open Research Fund of Key Laboratory for Organic Electronics and Information Displays

Publisher

MDPI AG

Subject

Clinical Biochemistry,General Medicine,Analytical Chemistry,Biotechnology,Instrumentation,Biomedical Engineering,Engineering (miscellaneous)

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