Physiological and Biochemical Behaviours and Antioxidant Response of Helianthus annuus under Lanthanum and Cerium Stress

Abstract

The continuous progress of global manufacturing and anthropogenic activities has resulted in excessive environmental metallic pollution, particularly with rare earth elements (REEs) which have become a prevalent issue of global concern due to their high toxicity and widespread existence. REEs-contaminated soils could ruin agriculture by inducing plant physiology disturbances in various crops that are considered the principal link of the human food chain. The main purpose of the present work is to assess the phytotoxicity of two light REEs, lanthanum (La) and cerium (Ce), in Helianthus annuus after 14 days of exposure to different concentrations of La and Ce (0, 1, 2.5, 5, and 10 µM). Plants showed different variations in shoot and root lengths at the end of the trial period. The accumulation of photosynthetic pigments, such as chlorophylls and carotenoids, as well as the photosynthetic efficiency, the non-photochemical quenching, the photosynthetically active radiation, and the electron transport rate, increased in the two REE treatments. Hydrogen peroxide significantly increased in all applied concentrations of La and Ce. A significant increase in malondialdehyde content was noticed only when plants were exposed to 2.5 µM La and 10 µM Ce. Results also demonstrated that La and Ce induced an increase in the activity of superoxide dismutase, peroxidase, and catalase (only the highest concentration of La decreased catalase activity). The exposure to different REE concentrations induced the accumulation of La and Ce in the plants, mainly in roots. Helianthus annuus showed an effective resistance behaviour facing La- and Ce-induced stresses.