Studies of Fluorescence Lifetimes of Biological Warfare Agents Simulants and Interferers Using the Stroboscopic Method

Author:

Kaliszewski Miron1ORCID,Kwaśny Mirosław1ORCID,Bombalska Aneta1ORCID,Włodarski Maksymilian1ORCID,Trafny Elżbieta Anna2ORCID,Kopczyński Krzysztof1

Affiliation:

1. Institute of Optoelectronics, Military University of Technology, Kaliskiego 2, 00-908 Warsaw, Poland

2. Biomedical Engineering Centre, Institute of Optoelectronics, Military University of Technology, Kaliskiego 2, 00-908 Warsaw, Poland

Abstract

The fluorescence decays (FDs) of 27 dried vegetative bacteria, bacterial endospores, fungi, and pollens were measured and determined using a stroboscopic technique. Pulsed nanosecond LED sources, emitting light at wavelengths of 280, 340, and 460 nm, were used for the excitation of biological samples. The implicit advantages of the stroboscopic method are high sensitivity, speed of a single measurement (10–60 s), miniaturization of the device, and relatively low price compared to the typical lifetime methods. The Principal Component Analysis (PCA) method was used for chemometric analysis. It was found that the excitation at 340, 460, and data merged from 340 and 460 nm effectively separate individual groups of biological substances. These findings provide evidence that fluorescence decay data may allow the classification of the biological samples, and the FDs measurement method can be complementary to the study of fluorescence spectra.

Funder

National Centre for Research and Development

Publisher

MDPI AG

Reference53 articles.

1. Primary biological aerosol particles in the atmosphere: A review;Burrows;Tellus Ser. B Chem. Phys. Meteorol.,2012

2. Non-culturable bioaerosols in indoor settings: Impact on health and molecular approaches for detection;Perrott;Atmos. Environ.,2015

3. Bioaerosols in the Earth system: Climate, health, and ecosystem interactions;Kampf;Atmos. Res.,2016

4. Doron, S., and Gorbach, S.L. (2008). Bacterial Infections: Overview. Int. Encycl. Public Health, 273–282.

5. CDC (2023, December 22). Bioterrorism Agents/Diseases, Available online: https://emergency.cdc.gov/agent/agentlist-category.asp.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3