Affiliation:
1. College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing 400715, China
2. Academy of Agricultural Sciences, Southwest University, Beibei, Chongqing 400715, China
Abstract
Plant height (PH) is a critical agronomic trait in Brassica napus, significantly impacting yield. Consequently, identifying genes associated with plant height is a pivotal objective in oilseed rape breeding. This study employed a combination of bulk segregant analysis sequencing (BSA-seq) and RNA sequencing (RNA-seq) for analysis. A novel quantitative trait locus (QTL), qPH_C02, was identified between 63,989,634 and 64,945,122 bp on chromosome C02, from which eight candidate genes were screened. The Gene Ontology (GO) analysis revealed enrichment in peroxisomes, while the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated enrichment in the oxidative phosphorylation (OP) pathway. It is hypothesized that the observed differences in plant height and silique length may be attributed to the regulation of peroxidase activity in the OP pathway, which in turn alters plant energy metabolism and controls nutrient uptake. Subsequently, we will further test this hypothesis. The results of this study will contribute to our understanding of the genetic basis for differences in plant height and provide a foundation for the selection and breeding of Brassica napus varieties with desired plant shapes.
Funder
the Department of Agriculture Projects of Modern Agricultural Technology System
Reference61 articles.
1. Detection of QTLs for plant height related traits in Brassica napus L. using DH and immortalized F2 population;He;Acta Agric. Sin.,2018
2. Sun, C.M., Wang, B.Q., Yan, L., Hu, K.N., Liu, S., Zhou, Y.M., Guan, C.Y., Zhang, Z.Q., Li, J.N., and Zhang, J.F. (2016). Genome-Wide association study provides insight into the genetic control of plant height in rapeseed (Brassica napus L.). Front. Plant Sci., 7.
3. Development of a SCAR (sequence characterised amplified region) marker for molecular tagging ofthe dwarf BREIZH (Bzh) gene in Brassica napus L.;Barret;Theor. Appl. Genet.,1998
4. Genetic analysis and characterization of hormone response of semi-dwarf mutant dw-1 in Brasscia napus L.;Song;Sci. Agric. Sin.,2019
5. Analysis of dwarf mutants induced by EMS in Brassica napus;Li;Life Sci. Res.,2020