Self-Assembled Amphiphilic Chitosan Nanomicelles: Synthesis, Characterization and Antibacterial Activity
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Published:2023-10-30
Issue:11
Volume:13
Page:1595
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ISSN:2218-273X
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Container-title:Biomolecules
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language:en
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Short-container-title:Biomolecules
Author:
Qi Yi123, Chen Qizhou1, Cai Xiaofen1, Liu Lifen1, Jiang Yuwei1, Zhu Xufeng12, Huang Zhicheng1, Wu Kefeng123, Luo Hui123, Ouyang Qianqian123ORCID
Affiliation:
1. Marine Biomedical Research Institution, Guangdong Medical University, Zhanjiang 524023, China 2. The Marine Biomedical Research Institute of Guangdong Zhanjiang, Zhanjiang 524023, China 3. The Key Lab of Zhanjiang for R&D Marine Microbial Resources in the Beibu Gulf Rim, Marine Biomedical Research Institute, Guangdong Medical University, Zhanjiang 524023, China
Abstract
Although amphiphilic chitosan has been widely studied as a drug carrier for drug delivery, fewer studies have been conducted on the antimicrobial activity of amphiphilic chitosan. In this study, we successfully synthesized deoxycholic acid-modified chitosan (CS-DA) by grafting deoxycholic acid (DA) onto chitosan C2-NH2, followed by grafting succinic anhydride, to prepare a novel amphiphilic chitosan (CS-DA-SA). The substitution degree was 23.93% for deoxycholic acid and 29.25% for succinic anhydride. Both CS-DA and CS-DA-SA showed good blood compatibility. Notably, the synthesized CS-DA-SA can self-assemble to form nanomicelles at low concentrations in an aqueous environment. The results of CS, CS-DA, and CS-DA-SA against Escherichia coli and Staphylococcus aureus showed that CS-DA and CS-DA-SA exhibited stronger antimicrobial effects than CS. CS-DA-SA may exert its antimicrobial effect by disrupting cell membranes or forming a membrane on the cell surface. Overall, the novel CS-DA-SA biomaterials have a promising future in antibacterial therapy.
Funder
Project of the Ph.D. Start-up Fund of Guangdong Medical University Project of Traditional Chinese Medical of Guangdong Province science and technology innovation team project of Guangdong Provincial medical product Administration Key Discipline Construction Project of Guangdong Medical University Special Funds for the Economic Development of the Marine Economy of Guangdong Province, China public service platform of the South China Sea for R&D marine biomedicine resources
Subject
Molecular Biology,Biochemistry
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