SCR Suppressor Mutants: Role in Hypocotyl Gravitropism and Root Growth in Arabidopsis thaliana

Abstract

The SCARECROW (SCR) transcription factor plays a key role in plant growth and development. However, we know very little about the role of SCR regulated pathways in plant development. Here, we used the homozygous scr1 mutant Arabidopsis thaliana (Wassilewskija ecotype), which had a T-DNA insertion in the SCR coding region and lacks a detectable SCR transcript. This scr1 mutant has a determinate mode of root growth, shoot agravitropism and abnormal internal architecture in all organs examined. To screen for mutants that suppress the scr1 abnormal phenotypes, we exposed homozygous scr1 seeds to ethyl methane sulphonate (EMS) mutagen. Upon growth out of these mutagenized seeds, thirteen suppressor mutant-harboring strains were identified. All thirteen suppressor-harboring strains were homozygous for scr1 and lacked the SCR transcript. Ten scr hypocotyl gravitropic suppressor lines showed improved hypocotyl gravitropic response. These ten suppressors fall into six complementation groups suggesting six different gene loci. Similarly, three independent scr root length suppressor lines rescued only the root growth phenotype and fell into three complementation groups, suggesting the involvement of three different gene loci. These suppressors might identify novel functions of the SCR gene in plant development.