Histopathology of Corn Plants Infected by Endophytic Fungi

Abstract

Endophytic fungi inhabiting plant tissues show extensive functional diversity, ranging from mutualism to pathogenicity. The present study evaluated the histological responses of corn plants to colonization by three species of endophytic fungi isolated from corn. Corn seedlings were inoculated with 1 × 106 conidia per mL spore suspensions of endophytic Fusarium verticillioides, Fusarium sacchari, and Penicillium citrinum and observed for 14 days for the emergence of disease symptoms. Histological examination of diseased root, stem, and leaf tissues was conducted using light and transmission electron microscopy. The results indicated that the mean diameters of root phloem, stem vascular bundles, and leaf vascular bundles, of corn plants infected with endophytic P. citrinum (18.91 µm, 146.96 µm, and 107.86 µm, respectively), F. verticillioides (18.75 µm, 85.45 µm, and 118.24 µm, respectively), and F. sacchari (24.15 µm root phloem, and 98.90 µm stem vascular bundle diameters), were significantly lower than the root phloem (33.68 µm), stem vascular bundle (186.77 µm), and leaf vascular bundle (155.88 µm) of the uninfected corn plants (p ≤ 0.05). Endophytic F. verticillioides was the most virulent, resulting in severe degradation and the eventual collapse of infected plant tissues. The study showed that endophytic fungi recovered from corn plants are capable of initiating significant disease responses in infected corn tissues.