Affiliation:
1. College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China
2. Jiangsu Academy of Forestry, Nanjing 211153, China
3. Jiangsu Yangzhou Urban Forest Ecosystem National Observation and Research Station, Yangzhou 225006, China
Abstract
The composition and diversity of the rhizosphere microbial community maintain the stability of the root microclimate, and several studies have focused on this aspect of rhizosphere microorganisms. However, how these communities vary with cultivars of a species is not completely understood. Paeonia lactiflora—a perennial herb species of the family Paeoniaceae—includes a wide variety of cultivars, with rich rhizosphere microbial resources. Hence, we studied the differences in rhizosphere bacterial communities associated with eight P. lactiflora cultivars. We noted that Actinobacteria, Proteobacteria, Acidobacteria, Bacteroidetes, Firmicutes, Verrucomicrobia, Planctomycetes and Chloroflexi were the dominant phyla associated with the cultivars. The composition of rhizosphere bacterial community of different cultivars was highly similar at taxonomic levels, but there were slightly differences in the relative abundance. LEfSe analysis showed that the cultivars “Sheng Tao Hua” and “Zi Lou Xian Jin” exhibited the most biomarkers. Differential ASV analysis revealed the maximum difference in ASV abundance between “Lian Tai” and “Zi Hong Zheng Hui”, as well as between “Sheng Tao Hua” and “Tao Hua Fei Xue”, and the maximum similarity between “Duo Ye Zi” and “Xue Feng”. Co-occurrence network analysis revealed that rhizosphere bacteria in most cultivars maintain homeostasis by cooperation, wherein Actinobacteria and Proteobacteria played a vital role. In addition, microbial resources related to cultivars like bioremediation, organic degradation and resistance to diseases are found. This study revealed the structures of the rhizosphere bacterial communities associated with different cultivars of P. lactiflora and explored their stress resistance potential, which can be used to guide future agricultural practices.
Funder
National Natural Science Foundation of China
Forestry Science and Technology Innovation and Promotion Project of Jiangsu Province
Construction Model of Efficient Farmland Protection Forest Network in Jiangsu Province
Efficiency Management Technology of Carbon Sequestration Forest in Jiangsu coast
Natural Science Foundation of Jiangsu Province, China
Subject
General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology