Abstract
Pyrethroids are widely applied insecticides in agriculture, but their frequent use has provoked many cases of resistance, in which mutations in the voltage-gated sodium channel (VGSC), the pyrethroid target-site, were shown to play a major role. However, for the spider mite Tetranychus urticae, it has also been shown that increased detoxification contributes to resistance against the pyrethroid bifenthrin. Here, we performed QTL-mapping to identify the genomic loci underlying bifenthrin resistance in T. urticae. Two loci on chromosome 1 were identified, with the VGSC gene being located near the second QTL and harboring the well-known L1024V mutation. In addition, the presence of an L925M mutation in the VGSC of a highly bifenthrin-resistant strain and its loss in its derived, susceptible, inbred line indicated the importance of target-site mutations in bifenthrin resistance. Further, RNAseq experiments revealed that genes encoding detoxification enzymes, including carboxyl/choline esterases (CCEs), cytochrome P450 monooxygenases and UDP-glycosyl transferases (UGTs), were overexpressed in resistant strains. Toxicity bioassays with bifenthrin (ester pyrethroid) and etofenprox (non-ester pyrethroid) also indicated a possible role for CCEs in bifenthrin resistance. A selection of CCEs and UGTs were therefore functionally expressed, and CCEinc18 was shown to metabolize bifenthrin, while teturUGT10 could glycosylate bifenthrin-alcohol. To conclude, our findings suggest that both target-site and metabolic mechanisms underlie bifenthrin resistance in T. urticae, and these might synergize high levels of resistance.
Funder
Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program
Subject
General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology
Reference122 articles.
1. Pyrethroids;Comprehensive Molecular Insect Science,2005
2. Perveen, F. (2012). Insecticides—Advances in Integrated Pest Management, InTech.
3. Van Leeuwen, T., Vontas, J., Tsagkarakou, A., and Tirry, L. (2009). Biorational Control of Arthropod Pests, Springer.
4. Global pesticide use: Profile, trend, cost / benefit and more;Proc. Int. Acad. Ecol. Environ. Sci.,2018
5. Insecticides, biologics and nematicides: Updates to IRAC’s mode of action classification—A tool for resistance management;Pestic. Biochem. Physiol.,2020
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