The Impact of Vp-Porin, an Outer Membrane Protein, on the Biological Characteristics and Virulence of Vibrio Parahaemolyticus-Reference-Cited by-同舟云学术

The Impact of Vp-Porin, an Outer Membrane Protein, on the Biological Characteristics and Virulence of Vibrio Parahaemolyticus

Published:2024-06-28 Issue:7 Volume:13 Page:485
ISSN:2079-7737
Container-title:Biology
language:en
Short-container-title:Biology

Author:

Che Jinyuan¹,Fang Qitong²,Hu Shaojie²,Liu Binghong²,Wang Lei¹,Fang Xiu³,Li Lekang⁴,Luo Tuyan⁵,Bao Baolong²

Affiliation:

1. Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China

2. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China

3. Fujian Provincial Key Laboratory of Breeding Lateolabrax Japonicus, Fuding 355200, China

4. Jiujiang Academy of Fishery Sciences, Jiujiang 332000, China

5. Institute of Quality Standards and Testing Technology for Agro-Products, Fujian Academy of Agricultural Science, Fuzhou 350003, China

Abstract

Porins are crucial proteins located in the outer membrane that directly influence antimicrobial resistance mechanisms and virulence in bacteria. In this study, a porin gene (Vp-porin) was cloned in V. parahaemolyticus, and the function of Vp-Porin in biological characteristics and virulence was investigated. The results of sequence analysis showed that Vp-Porin is highly conserved in Vibrio spp., and the predicted 3D structure showed it could form a 20-strand transmembrane β-barrel domian. Membrane permeabilization provides evidence that the membrane integrity of ∆Vp-porin was damaged and the sensitivity to tetracycline, polymyxin B, rifampicin and cephalothin of ∆Vp-porin obviously increased. In addition, loss of Vp-porin damaged motility due to downregulated flagellar synthesis. In addition, ∆Vp-porin exhibited attenuated cytotoxicity to Tetrahymena. The relative survival rate of Tetrahymena infection with ∆Vp-porin was 86%, which is much higher than that with WT (49%). Taken together, the results of this study indicate that Vp-Porin in V. parahaemolyticus plays various roles in biological characteristics in membrane integrity, antimicrobial resistance and motility and contributes to virulence.

Funder

National Key R&D Program of China

Regional Development Project of Fujian

Fisheries High Quality Development Project of Fujian

Publisher

MDPI AG

Link

https://www.mdpi.com/2079-7737/13/7/485/pdf

Reference39 articles.

1. Vibrio Parahaemolyticus: A Review on Distribution, Pathogenesis, Virulence Determinants and Epidemiology;Ghenem;Saudi J. Med. Med. Sci.,2017

2. Molecular mechanisms of Vibrio parahaemolyticus pathogenesis;Li;Microbiol. Res.,2019

3. Xu, X., Cheng, J., Wu, Q., Zhang, J., and Xie, T. (2016). Prevalence, characterization, and antibiotic susceptibility of Vibrio parahaemolyticus isolated from retail aquatic products in North China. BMC Microbiol., 16.

4. The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus;Lu;J. Microbiol.,2021

5. Haifa-Haryani, W.O., Amatul-Samahah, M.A., Azzam-Sayuti, M., Chin, Y.K., Zamri-Saad, M., Natrah, I., Amal, M.N.A., Satyantini, W.H., and Ina-Salwany, M.Y. (2022). Prevalence, Antibiotics Resistance and Plasmid Profiling of Vibrio spp. Isolated from Cultured Shrimp in Peninsular Malaysia. Microorganisms, 10.