Evaluation of Teneligliptin and Retagliptin on the Clearance of Melanosome by Melanophagy in B16F1 Cells

Author:

Kim Seong Hyun1,Bae Ji-Eun2,Park Na Yeon1,Kim Joon Bum1,Kim Yong Hwan1,Kim So Hyun1,Oh Gyeong Seok1,Wang Hee Won1,Chang Jeong Ho3ORCID,Cho Dong-Hyung14

Affiliation:

1. BK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea

2. KNU LAMP Research Center, Institute of Basic Sciences, Kyungpook National University, Daegu 41566, Republic of Korea

3. Department of Biology Education, Kyungpook National University, Daegu 41566, Republic of Korea

4. Organelle Institute, Kyungpook National University, Daegu 41566, Republic of Korea

Abstract

A specialized membrane-bound organelle, named the melanosome, is central to the storage and transport of melanin as well as melanin synthesis in melanocytes. Although previous studies have linked melanosomal degradation to autophagy, the precise mechanisms remain elusive. Autophagy, a complex catabolic process involving autophagosomes and lysosomes, plays a vital role in cellular constituent degradation. In this study, the role of autophagy in melanosomal degradation was explored, employing a cell-based screening system designed to unveil key pathway regulators. We identified specific dipeptidyl peptidase-4 inhibitors, such as teneligliptin hydrobromide and retagliptin phosphate, as novel agents inducing melanophagy through a comprehensive screening of a ubiquitination-related chemical library. We found that treatment with teneligliptin hydrobromide or retagliptin phosphate not only diminishes melanin content elevated by alpha-melanocyte-stimulating hormone (α-MSH) but also triggers autophagy activation within B16F1 cells. In addition, the targeted inhibition of unc-51-like kinase (ULK1) significantly attenuated both the anti-pigmentation effects and autophagy induced by teneligliptin hydrobromide and retagliptin phosphate in α-MSH-treated cells. Collectively, our data demonstrate a new frontier in understanding melanosomal degradation, identifying teneligliptin hydrobromide and retagliptin phosphate as promising inducers of melanophagy via autophagy activation. This study contributes essential insights into cellular degradation mechanisms and offers potential therapeutic avenues in the regulation of pigmentation.

Funder

National Research Foundation of Korea

Korea Institute for Advancement of Technology funded by the Ministry of Trade, Industry and Energy

ORGASIS corporation

Publisher

MDPI AG

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