Wax-Printed Fluidic Controls for Delaying and Accelerating Fluid Transport on Paper-Based Analytical Devices

Abstract

In this work, we explore a new method for controlling fluid transport rate on paper-based analytical devices that enables both the delay and the acceleration of fluid flow. The delays were incorporated by wax printing linear patterns of variable width within the flow channel and melted to penetrate the paper. In this manner, the surface tension of the fluid decreases while its contact angle increases, causing a pressure drop along the fluid path that reduces capillary flow. The acceleration of flow was accomplished by overlaying hydrophobic stripes (prepared by wax printing and melting the wax) on the hydrophilic path (top or top–bottom). In this manner, the fluid was repelled from two dimensions (vertical and applicate), increasing the flow rate. The combination of these methods on the same devices could adjust wicking time in intermediate time internals. The method enabled a wide timing of fluid transport, accomplishing a change in wicking times that extended from −41% to +259% compared to open paper channels. As a proof of concept, an enzymatic assay of glucose was used to demonstrate the utility of these fluid control methods in kinetic methods of analysis.