Affiliation:
1. Institut für Bioprozess- und Analysenmesstechnik e.V., 37308 Heilbad Heiligenstadt, Germany
Abstract
Biofilms are ubiquitous at interfaces of natural and technical origin. Depending on type and application, biofilm formation is desired or has to be prevented. Therefore, reliable detection of initial biofilm growth is essential in many areas. One method of biofilm monitoring is the electrochemical impedance spectroscopy. Among other factors, this method is heavily dependent on the electrode geometry. In order to achieve a high measurement sensitivity, the electrode size must be chosen according to the biofilm that is to be measured. This paper presents an approach for simulating and modeling the optimal electrode geometry for a specific biofilm. First, a geometric model of a biofilm with up to 6000 individual bacteria is generated. The simulated impedances are used to calculate which electrode geometry maximizes sensitivity depending on the biofilm height. In the chosen example of an E. coli biofilm in a nutrient solution, the optimum size of an interdigital electrode (bar gap equals width) was 2.5 µm for a biofilm height of up to 2 µm. The used algorithms and models can be simply adapted for other biofilms. In this way, the most sensitive electrode geometry for a specific biofilm measurement can be determined with minimal effort.
Funder
Deutsche Forschungsgemeinschaft
Subject
Physical and Theoretical Chemistry,Analytical Chemistry
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