Nitrogen-Doped Graphene-Based Sensor for Electrochemical Detection of Piroxicam, a NSAID Drug for COVID-19 Patients

Abstract

Nitrogen-doped graphene (NGr) was synthesized by the hydrothermal method using urea as a reducing and doping agent for graphene oxide (GO). The crystalline structure of GO was revealed by the XRD intense peak recorded at 2θ = 11.4°, indicating that the interlayer distance within the structure was large (d = 0.77 nm), and the number of layers (n) was 9. Further, the transformation of GO in NGr also led to the decrease in the interlayer distance and number of layers (d = 0.387 nm; n = 3). As indicated by elemental analysis, the concentration of nitrogen in the NGr sample was 6 wt%. Next, the comparison between the performance of bare GC and the graphene-modified electrode (NGr/GC) towards piroxicam (PIR) detection was studied. Significant differences were observed between the two electrodes. Hence, in the case of bare GC, the oxidation signal of PIR was very broad and appeared at a high potential (+0.7 V). In contrast, the signal recorded with the NGr/GC electrode was significantly higher (four times) and shifted towards lower potentials (+0.54 V), proving the electro-catalytic effect of nitrogen-doped graphene. The NGr/GC electrode was also tested for its ability to detect piroxicam in pharmaceutical drugs (Flamexin), giving excellent recoveries.