Assessment of Oxidative Stress by Detection of H2O2 in Rye Samples Using a CuO- and Co3O4-Nanostructure-Based Electrochemical Sensor
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Published:2023-10-10
Issue:10
Volume:11
Page:532
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ISSN:2227-9040
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Container-title:Chemosensors
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language:en
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Short-container-title:Chemosensors
Author:
Mihailova Irena1ORCID, Krasovska Marina1ORCID, Sledevskis Eriks1ORCID, Gerbreders Vjaceslavs1ORCID, Mizers Valdis1ORCID, Ogurcovs Andrejs12ORCID
Affiliation:
1. G. Liberts’ Innovative Microscopy Centre, Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, Parades Street 1a, LV-5401 Daugavpils, Latvia 2. Institute of Solid State Physics, University of Latvia, Kengaraga Street 8, LV-1063 Riga, Latvia
Abstract
Hydrogen peroxide is essential for biological processes and normally occurs in low concentrations in living organisms. However, exposure of plants to biotic and abiotic stressors can disrupt their defense mechanisms, resulting in oxidative stress with elevated H2O2 levels. This oxidative stress can damage cell membranes, impair photosynthesis, and hinder crucial plant functions. The primary focus of this article is to investigate the effects of salt and herbicide stress factors on the growth of rye samples. For precise quantification of the released H2O2 concentration caused by these stress factors, a non-enzymatic electrochemical sensor was developed, employing nanostructured CuO and Co3O4 oxides. Nanostructured electrodes exhibit high sensitivity and selectivity towards H2O2, making them suitable for detecting H2O2 in real samples with complex compositions. Rye samples exposed to NaCl- and glyphosate-induced stress demonstrated notable concentrations of released H2O2, displaying an increase of up to 30% compared to the control sample. Moreover, optical absorption measurements revealed a substantial decrease in chlorophyll concentration (up to 35% compared to the control group) in rye samples where elevated H2O2 levels were detected through electrochemical methods. These findings provide further evidence of the harmful effects of elevated H2O2 concentrations on plant vital functions.
Funder
European Regional Development Fund Activity 1.1.1.2 “Post-doctoral Research Aid”
Subject
Physical and Theoretical Chemistry,Analytical Chemistry
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