Effects of Obesity and Diabesity on Ventricular Muscle Structure and Function in the Zucker Rat

Author:

Sultan AhmedORCID,Adeghate ErnestORCID,Emerald Bright StarlingORCID,Qureshi Muhammad A.,Minhas Saeed Tariq,Howarth Frank Christopher

Abstract

(1) Background: Cardiovascular complications are a leading cause of morbidity and mortality in diabetic patients. The effects of obesity and diabesity on the function and structure of ventricular myocytes in the Zucker fatty (ZF) rat and the Zucker diabetic fatty (ZDF) rat compared to Zucker lean (ZL) control rats have been investigated. (2) Methods: Shortening and intracellular Ca2+ were simultaneously measured with cell imaging and fluorescence photometry, respectively. Ventricular muscle protein expression and structure were investigated with Western blot and electron microscopy, respectively. (3) Results: The amplitude of shortening was increased in ZF compared to ZL but not compared to ZDF myocytes. Resting Ca2+ was increased in ZDF compared to ZL myocytes. Time to half decay of the Ca2+ transient was prolonged in ZDF compared to ZL and was reduced in ZF compared to ZL myocytes. Changes in expression of proteins associated with cardiac muscle contraction are presented. Structurally, there were reductions in sarcomere length in ZDF and ZF compared to ZL and reductions in mitochondria count in ZF compared to ZDF and ZL myocytes. (4) Conclusions: Alterations in ventricular muscle proteins and structure may partly underlie the defects observed in Ca2+ signaling in ZDF and ZF compared to ZL rat hearts.

Funder

Zayed Center for Health Sciences, United Arab Emirates University

Publisher

MDPI AG

Subject

Paleontology,Space and Planetary Science,General Biochemistry, Genetics and Molecular Biology,Ecology, Evolution, Behavior and Systematics

Reference58 articles.

1. IDF Diabetes Atlas,2021

2. Obesity and Overweight https://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight

3. World Stroke Organization Global Atlas on Cardiovascular Disease Prevention and Control,2011

4. Hypoadiponectinemia in Obesity and Type 2 Diabetes: Close Association with Insulin Resistance and Hyperinsulinemia

5. Global and societal implications of the diabetes epidemic

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