Establishment and Characterization of a Primary Fibroblast Cell Culture from the Amazonian Manatee (Trichechus inunguis)
Author:
Tavares Flávia dos Santos1, Martins Cesar2ORCID, Delella Flávia Karina2, Nascimento Luís Adriano Santos do3ORCID, Rodrigues Angélica Lúcia Figueiredo4, Moreira Sávia4, Cardoso Adauto Lima123ORCID, Noronha Renata Coelho Rodrigues1ORCID
Affiliation:
1. Laboratório de Genética e Biologia Celular, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, PA, Brazil 2. Department of Structural and Functional Biology, Institute of Biosciences at Botucatu, Sao Paulo State University—UNESP, Botucatu 18618-689, SP, Brazil 3. Laboratório de Óleos da Amazônia, Universidade Federal do Pará, Belém 66075-110, PA, Brazil 4. Instituto Biologia e Conservação dos Mamíferos Aquáticos da Amazônia (BioMA), Belém 66077-830, PA, Brazil
Abstract
The vulnerable status of the Amazon manatee, Trichechus inunguis, indicates the need to seek measures to guarantee its conservation. In this context, the cultivation of cells in vitro is a strategy that should at least guarantee the preservation of their genetic material. Thus, we established for the first time a primary culture of Amazonian manatee fibroblasts (TINsf) from a skin biopsy of a young male. Karyotypic analysis of the 3rd, 7th, and 12th passages confirmed the taxonomic identity of the species T. inunguis (2n = 56/NF = 92) and indicated that this culture presents genomic stability. Gene and protein expression of vimentin at the 13th passage show the predominant presence of fibroblasts in TINsf. To test the cell line’s responsiveness to materials and demonstrate a possible application of this culture, it was exposed to andiroba seed oil (ASO), and its viability and proliferative capacity were evaluated. ASO demonstrated toxic effects at the highest concentrations and longest exposure times tested, reproducing results observed in human cultures, indicating the applicability of TINsf in toxicological and biotechnological studies. After cryopreservation, the TINsf line maintained its proliferative potential, indicating the establishment of a new culture available for future studies.
Funder
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Conselho Nacional de Desenvolvimento Científico e Tecnológico São Paulo Research Foundation Fundação Amazônia de Amparo a Estudos e Pesquisas
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2 articles.
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