Affiliation:
1. Department of Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine, Faculty of Pharmacy, University of Valencia, Burjassot, 46100 Valencia, Spain
Abstract
The bioaccessibility of mycotoxins is an important factor that has to be considered when assessing the risk they pose to human health. Bioactive compounds like phenolics could play a protective role against the toxic effects of contaminants. In this work, the bioaccessible fraction of the T-2 toxin (T-2) contained in breakfast cereals and its effect on the viability of Caco-2 cells were investigated. Furthermore, the effect of tyrosol (a polyphenol abundant in EVOO) on T-2-induced cytotoxicity was evaluated in the same cell line. After standardized in vitro gastrointestinal digestion, the T-2 toxin was released from T-2-spiked breakfast cereals and further quantified by UHPLC-MS/MS. The bioaccessible fraction of T-2 was 51 ± 4%. The cell viability study was performed by pre-treating the cells for 24 h with tyrosol (25, 50 and 100 µM) and subsequently adding T-2 at 15 nM or by treating the cells with a combination of tyrosol and T-2. In the simultaneous treatment, 25 µM tyrosol prevented the toxic effects produced by the exposure to T-2 at 15 nM; however, cytotoxic effects were observed for the other combinations tested. The pre-treatment of Caco-2 cells with tyrosol did not attenuate the cytotoxic effects caused by exposure to T-2. These results suggest that tyrosol at low concentrations (25 µM) could exert a cytoprotective effect on Caco-2 cells against 15 nM T-2 when administered simultaneously with T-2. However, more studies are required to corroborate this hypothesis.
Funder
Spanish Ministry of Science and Innovation
Subject
Health, Toxicology and Mutagenesis,Toxicology
Reference32 articles.
1. Health endorsing perspective of cereals and its by products: A systematic review;Amjad;Biosci. Res.,2021
2. Occurrence and fate of mycotoxins in cereals and cereal-based products: A narrative review of systematic reviews and meta-analyses studies;Sarmast;Curr. Opin. Food Sci.,2021
3. Liu, L., Xie, M., and Wei, D. (2022). Biological detoxification of mycotoxins: Current status and future advances. Int. J. Mol. Sci., 23.
4. RASFF (Rapid Alert for Food and Feed) (2022, September 29). RASFF Annual Report 2021. Available online: https://food.ec.europa.eu/system/files/2022-07/acn_annual-report_2021-final.pdf.
5. EFSA (European Food Safety Authority) (2017). Scientific report on Human and animals dietary exposure to T-2 and HT-2 toxin. EFSA J., 15, 4972.
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