Characteristics of Chromophoric Dissolved Organic Matter (CDOM) Produced by Heterotrophic Bacteria Isolated from Aquaculture Systems

Abstract

Heterotrophic bacteria (HB) play an important role in aquatic ecosystems as recyclers of dissolved organic matter (DOM). The objective of this study was to characterize the spectral characteristics of intracellular (IC), and extracellular (EC) compounds produced by 12 HB isolated from two aquaculture systems. Microorganisms belonging to the genera Bacillus, Paenibacillus, and Psychrobacillus were identified by analysis of the 16S ribosomal gene. Aliquots of bacterial culture were centrifugated every hour (1st to 7th) to obtain the EC compounds. The pellet was ultrasound-lysed to obtain the IC compounds. Excitation-emission matrices were used in combination with parallel factor analysis (PARAFAC) to characterize the fluorescent components of DOM (FDOM). PARAFAC indicated two protein-like components and two humic-like components in both cell spaces. At the IC, B. macquariensis showed a high fluorescence index (FI), probably associated with fulvic acid, quinones, or ketones. Psychrobacillus insolitus showed an inverse correlation between spectral slopes S275–295 and S350–400 in the EC and IC fractions, which may indicate differential release of low and high molecular weight molecules in these two fractions. The opposite occurred with B. licheniformis and P. alvei. The origin of FDOM in HB is an important finding of this work. The most significant amount of protein-like substances was produced at the IC level, with the humic- and fulvic-type at the EC. The main finding of this work is the evidence of differential production of humic-type or protein-type FDOM production by HB species from marine and freshwater aquaculture systems in their intracellular and extracellular fractions, as well different relative molecular weight. For aquaculture, these findings suggest that some bacterial species show promise in supplying essential amino acids to growing organisms, and others play a major role in nutrient exchange and the global carbon cycle.