Abstract
Infections with the opportunistic Gram-negative bacterium Acinetobacter baumannii pose a serious threat today, which is aggravated by the growing problem of multi-drug resistance among bacteria, caused by the overuse of antibiotics. Treatment of infections caused by antibiotic-resistant A. baumannii strains with the use of phage therapy is not only a promising alternative, but sometimes the only option. Therefore, phages specific for clinical multi-drug resistant A. baumannii were searched for in environmental, municipal, and hospital wastewater samples collected from different locations in Poland. The conducted research allowed us to determine the biological properties and morphology of the tested phages. As a result of our research, 12 phages specific for A. baumannii, 11 of which turned out to be temperate and only one lytic, were isolated. Their lytic spectra ranged from 11 to 75%. The plaques formed by most phages were small and transparent, while one of them formed relatively large plaques with a clearly marked ‘halo’ effect. Based on Transmission Electron Microscopy (TEM), most of our phages have been classified as siphoviruses (only one phage was classified as a podovirus). All phages have icosahedral capsid symmetry, and 11 of them have a long tail. Optimal multiplicity of infections (MOIs) and the adsorption rate were also determined. MOI values varied depending on the phage—from 0.001 to 10. Based on similarities to known bacteriophages, our A. baumannii-specific phages have been proposed to belong to the Beijerinckvirinae and Junivirinae subfamilies. This study provides an additional tool in the fight against this important pathogen and may boost the interest in phage therapy as an alternative and supplement to the current antibiotics.
Funder
Optimization of the production and characterization of bacteriophage preparations for therapeutic use” funded funds under Operational Program Innovative Economy
Biological stabilization of the microbiology of water intended for consumption project financed by the National Center for Research and Development
National Center for Research and Development
Subject
Virology,Infectious Diseases
Reference67 articles.
1. Acinetobacter: An old friend, but a new enemy;Towner;J. Hosp. Infect.,2009
2. Progress Against Antimicrobial Resistance Has Slipped;Kuehn;JAMA,2022
3. Genomic analysis of sewage from 101 countries reveals global landscape of antimicrobial resistance;Munk;Nat. Commun.,2022
4. (2018, November 14). Available online: http://www.who.int/mediacentre/news/releases/2017/bacteria-antibiotics-needed/en.
5. (2019, February 18). Available online: https://www.who.int/emergencies/ten-threats-to-global-health-in-2019.
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