HT-29 Colon Cancer Cell Electromanipulation and Assessment Based on Their Electrical Properties

Abstract

This study proposes a feasible approach for the rapid, sensitive, and label-free identification of cancerous cells based on dielectrophoretic (DEP) manipulation and electrical characterization. In this method, the concentration of target cells at the level of customized microelectrodes via DEP is first determined, followed by an electrical impedance evaluation. The study demonstrates the capacity of the methodology to electrically differentiate HT-29 cancer cells from healthy blood cells based on their impedance spectra. Within a higher frequency domain, the electrical impedance of trapped cancer cells was significantly lower compared with the normal ones. In order to evaluate the functionality and reproducibility of the proposed method, the influence of the DEP and EIS (electrical impedance spectroscopy) operating voltages on the electrical characterization of trapped HT-29 cells was analyzed.