Abstract
The development of new techniques for the detection of microRNAs (miRNAs) is highly desirable. Herein, a new crystalline three-component covalent organic framework (COF) termed EB-TAPB-TFP COF was synthesized under solvothermal conditions utilizing 1,3,5-triformylphloroglucinol, 1,3,5-tris(4-aminophenyl)benzene and ethidium bromide as monomers. Interestingly, EB-TAPB-TFP COF can be self-exfoliated into two-dimensional nanosheets (NSs) in an aqueous medium. The obtained EB-TAPB-TFP NSs exhibited a remarkable fluorescence intensity enhancement in the presence of a DNA-miRNA heteroduplex when compared to the presence of single-stranded DNA and other phosphate-based small molecules, making it promising in the detection of miRNA without tagging any fluorescent marker. Moreover, the EB-TAPB-TFP NSs can also be used as sensing material for the detection of a DNA-miRNA heteroduplex using the quartz crystal microbalance technique, which is in good agreement with the fluorescence sensing result. The exploration of COF-based sensors in this work demonstrates a new pathway for the selective detection of miRNAs.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Guangdong Province
Science and Technology Program of Guangzhou
Subject
Inorganic Chemistry,Condensed Matter Physics,General Materials Science,General Chemical Engineering
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