Affiliation:
1. Laboratoire de Santé Animale, ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, F-94700 Maisons-Alfort, France
Abstract
Ticks and tick-borne diseases (TBDs) are increasingly recognized as a critical One Health concern. Tick-borne encephalitis (TBE), a severe neuro infection caused by the tick-borne encephalitis virus (TBEV), has emerged as a significant global public health threat. Laboratory animals, particularly mice, have played a pivotal role in advancing our understanding of TBD pathogenesis. Notably, BALB/c mice have been employed as models due to their heightened susceptibility to TBEV. However, the use of C3H mice, valued for other tick-borne pathogens, has remained unexplored for TBEV until now. This study aimed to assess the susceptibility of C3H mice to TBEV infection, laying the groundwork for future co-infection models involving TBEV and Borrelia. Experiments revealed that C3H mice are susceptible to TBEV infection through subcutaneous inoculation. While 102 PFU/mouse appeared necessary for full infection, 103 PFU/mouse induced consistent symptoms. However, subsequent assessment of ticks’ acquisition of TBEV from infected mice met with limited success, raising questions about optimal infectious doses for natural infection. These findings suggest the potential of C3H mice for studying TBEV and co-infections with other pathogens, particularly Borrelia. Further exploration of the interplay between these pathogens, their transmission dynamics, and disease severity could enhance prevention and control strategies.
Funder
French Agency for Food, Environmental and Occupational Health & Safety
French National Institute for Agricultural Research
Ecole nationale vétérinaire d’Alfort
French Government’s Investissement d’Avenir program, Laboratoire d’Excellence “Integrative Biology of Emerging Infectious Diseases”
Subject
Virology,Infectious Diseases
Reference32 articles.
1. The global importance of ticks;Jongejan;Parasitology,2004
2. Boelke, M., Puff, C., Becker, K., Hellhammer, F., Gusmag, F., Marks, H., Liebig, K., Stiasny, K., Dobler, G., and Baumgärtner, W. (2021). Enteric ganglioneuritis, a common feature in a subcutaneous tbev murine infection model. Microorganisms, 9.
3. Tick-borne encephalitis;Gritsun;Med. Ther. Pediatr.,2015
4. Tick-borne encephalitis in Europe and Russia: Review of pathogenesis, clinical features, therapy, and vaccines;Ruzek;Antiviral. Res.,2019
5. Rodents as sentinels for the prevalence of tick-borne encephalitis virus;Achazi;Vector-Borne Zoonotic Dis.,2011
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献