Bioluminescence Inhibition Bioassay for Estimation of Snow Cover in Urbanised Areas within Boreal Forests of Krasnoyarsk City

Author:

Rimashevskaya Anastasia A.1,Muchkina Elena Y.1,Sutormin Oleg S.12ORCID,Chuyashenko Dmitry E.1,Gareev Arsen R.1,Tikhnenko Svetlana A.3,Rimatskaya Nadezhda V.4,Kratasyuk Valentina A.13

Affiliation:

1. School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk 660041, Russia

2. Department of Chemistry, Institute of Nature and Technical Sciences, Surgut State University, Surgut 628412, Russia

3. Photobiology Laboratory, Institute of Biophysics, Federal Research Center ‘Krasnoyarsk Science Center, Siberian Branch of the Russian Academy of Sciences’, Krasnoyarsk 660036, Russia

4. Department of Education Quality Management, Surgut State University, Surgut 628412, Russia

Abstract

It has been proposed that the level of air pollution in a city should be estimated based on the accumulation of pollutants in the snow cover of urban forests. This study presents a bioluminescence method for estimating the extent of snow cover pollution in the urbanised areas of boreal forests in Krasnoyarsk city. A bioluminescent assay involving NAD(P)H:FMN oxidoreductase (Red) and luciferase with luminous bacteria (BLuc) was employed to measure the concentrations of six heavy metals (As, Cd, Zn, Co, Hg, and Pb) in the snow cover. The tested snow samples demonstrated a correlation between the reduced activity of the enzyme system and variations in Cd concentration. Furthermore, the research indicated that the period of unfavourable meteorological conditions in Krasnoyarsk city resulted in a notable decline in the activity of the BLuc–Red enzyme system, which may be associated with elevated air pollution levels. This study underscores the potential of the bioluminescence method for monitoring environmental pollution in urban forested areas.

Funder

Russian Science Foundation

State Assignment of the Ministry of Science and Higher Education of the Russian Federation

Publisher

MDPI AG

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