Abstract
Zinc finger E-box-binding homeobox 1 (ZEB1) and zinc finger E-box-binding homeobox 2 (ZEB2) are transcription factors that regulate epithelial–mesenchymal transformation (EMT). The aim of this study was to compare levels of ZEB1 and ZEB2 in the peritoneal fluid and plasma between patients with and without endometriosis in order to assess their utility in the diagnostic process. Plasma and peritoneal fluid samples were collected from 50 patients with and 48 without endometriosis during planned surgical procedures in eight clinical centers. Quantitative ZEB1 and ZEB2 levels analyses were performed using a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). No significant differences were observed in ZEB1 levels in any of the subanalyses nor any differences regarding ZEB2 levels between patients with and without endometriosis. Plasma ZEB2 levels were significantly higher among patients with infertility compared to fertile women (16.07 ± 12.70 ng/L vs. 12.07 ± 11.92 ng/L; p < 0.04). Both ZEB1 and ZEB2 do not seem to have a significant value in the initial diagnosis of endometriosis as a single marker. The differences in ZEB2 plasma levels between patients with and without infertility indicate the possibility of EMT dysregulation in the pathogenesis of adverse fertility outcomes.
Funder
Polish Ministry of Health
Medical University of Warsaw
the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement
Subject
General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)
Reference56 articles.
1. Endometriosis and nuclear receptors;Yilmaz;Hum. Reprod. Update,2019
2. Endometriosis;Giudice;Lancet,2004
3. Peritoneal endometriosis due to the menstrual dissemination of endometrial tissue into the peritoneal cavity;Sampson;Am. J. Obstet. Gynecol.,1927
4. Endometriosis;Zondervan;N. Engl. J. Med.,2020
5. Załęcka, J., Pankiewicz, K., Issat, T., and Laudański, P. Molecular Mechanisms Underlying the Association between Endometriosis and Ectopic Pregnancy. Int. J. Mol. Sci., 2022. 23.
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献