Chitosan Nanoparticles Loaded with Quercetin and Valproic Acid: A Novel Approach for Enhancing Antioxidant Activity against Oxidative Stress in the SH-SY5Y Human Neuroblastoma Cell Line

Author:

Canbolat Fadime1,Demir Neslihan2ORCID,Yayıntas Ozlem Tonguc3ORCID,Pehlivan Melek4,Eldem Aslı5,Ayna Tulay Kilicaslan56ORCID,Senel Mehmet78ORCID

Affiliation:

1. Department of Pharmacy Services, Vocational School of Health Services, Çanakkale Onsekiz Mart University, Çanakkale 17100, Türkiye

2. Faculty of Science, Çanakkale Onsekiz Mart University, Çanakkale 17100, Türkiye

3. Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale 17100, Türkiye

4. Vocational School of Health Services, İzmir Katip Çelebi University, İzmir 35620, Türkiye

5. Medical Biology Department, Faculty of Medicine, İzmir Katip Çelebi University, İzmir 35620, Türkiye

6. Tissue Typing Laboratory, İzmir Tepecik Education and Research Hospital, İzmir 35180, Türkiye

7. Department of Biochemistry, Faculty of Pharmacy, Biruni University, Istanbul 34010, Türkiye

8. Department of Pharmaceutical Sciences, School of Pharmacy, University of California-Irvine, Irvine, CA 92697, USA

Abstract

Background: Multiple drug-delivery systems obtained by loading nanoparticles (NPs) with different drugs that have different physicochemical properties present a promising strategy to achieve synergistic effects between drugs or overcome undesired effects. This study aims to develop a new NP by loading quercetin (Que) and valproic acid (VPA) into chitosan. In this context, our study investigated the antioxidant activities of chitosan NPs loaded with single and dual drugs containing Que against oxidative stress. Method: The synthesis of chitosan NPs loaded with a single (Que or VPA) and dual drug (Que and VPA), the characterization of the NPs, the conducting of in vitro antioxidant activity studies, and the analysis of the cytotoxicity and antioxidant activity of the NPs in human neuroblastoma SH-SY5Y cell lines were performed. Result: The NP applications that protected cell viability to the greatest extent against H2O2-induced cell damage were, in order, 96 µg/mL of Que-loaded chitosan NP (77.30%, 48 h), 2 µg/mL of VPA-loaded chitosan NP (70.06%, 24 h), 96 µg/mL of blank chitosan NP (68.31%, 48 h), and 2 µg/mL of Que- and VPA-loaded chitosan NP (66.03%, 24 h). Conclusion: Our study establishes a successful paradigm for developing drug-loaded NPs with a uniform and homogeneous distribution of drugs into NPs. Chitosan NPs loaded with both single and dual drugs possessing antioxidant activity were successfully developed. The capability of chitosan NPs developed at the nanometer scale to sustain cell viability in SH-SY5Y cell lines implies the potential of intranasal administration of chitosan NPs for future studies, offering protective effects in central nervous system diseases.

Funder

Çanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit

Publisher

MDPI AG

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