CircAFF1 Is a Circular RNA with a Role in Alveolar Rhabdomyosarcoma Cell Migration
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Published:2023-07-04
Issue:7
Volume:11
Page:1893
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ISSN:2227-9059
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Container-title:Biomedicines
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language:en
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Short-container-title:Biomedicines
Author:
Centrón-Broco Alvaro1ORCID, Rossi Francesca2, Grelloni Chiara1, Garraffo Raffaele1, Dattilo Dario1ORCID, Giuliani Andrea1ORCID, Di Timoteo Gaia1, Colantoni Alessio13ORCID, Bozzoni Irene13ORCID, Beltran Nebot Manuel1ORCID
Affiliation:
1. Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, 00185 Rome, Italy 2. The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK 3. Center for Life Nano- & Neuro-Science, Fondazione Istituto Italiano di Tecnologia (IIT), 00161 Rome, Italy
Abstract
Circular RNAs (circRNAs), covalently closed RNAs that originate from back-splicing events, participate in the control of several processes, including those that occur in the development of pathological conditions such as cancer. Hereby, we describe circAFF1, a circular RNA overexpressed in alveolar rhabdomyosarcoma. Using RH4 and RH30 cell lines, a classical cell line models for alveolar rhabdomyosarcoma, we demonstrated that circAFF1 is a cytoplasmatic circRNA and its depletion impacts cell homeostasis favouring cell migration through the downregulation of genes involved in cell adhesion pathways. The presented data underline the importance of this circular RNA as a new partial suppressor of the alveolar rhabdomyosarcoma tumour progression and as a putative future therapeutic target.
Funder
ERC-2019-SyG AIRC PRIN 2017 H2020 Program “Sapienza Progetti Collaborativi” European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie
Subject
General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)
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