Protective Effects of Laminaria japonica Polysaccharide Composite Microcapsules on the Survival of Lactobacillus plantarum during Simulated Gastrointestinal Digestion and Heat Treatment

Author:

Guo Honghui123ORCID,Zhou Yelin14,Xie Quanling123ORCID,Chen Hui123ORCID,Zhang Ming’en1,Yu Lei2,Yan Guangyu2,Chen Yan5,Lin Xueliang5,Zhang Yiping123,Hong Zhuan123

Affiliation:

1. Engineering Technology Innovation Center for the Development and Utilization of Marine Living Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China

2. Xiamen Ocean Vocational College, Xiamen 361100, China

3. Fujian Key Laboratory of Island Monitoring and Ecological Development, Island Research Center, Ministry of Natural Resources, Pingtan 350400, China

4. College of Advanced Manufacturing, Fuzhou University, Quanzhou 362200, China

5. Haijia Flour Milling Company Limited, China Oil & Foodstuffs Corporation, Xiamen 361026, China

Abstract

To improve probiotics’ survivability during gastrointestinal digestion and heat treatment, Lactobacillus plantarum was microencapsulated by spray-drying using Laminaria japonica polysaccharide/sodium caseinate/gelatin (LJP/SC/GE) composites. Thermogravimetry and differential scanning calorimetry results revealed that the denaturation of LJP/SC/GE microcapsules requires higher thermal energy than that of SC/GE microcapsules, and the addition of LJP may improve thermal stability. Zeta potential measurements indicated that, at low pH of the gastric fluid, the negatively charged LJP attracted the positively charged SC/GE, helping to maintain an intact microstructure without disintegration. The encapsulation efficiency of L. plantarum-loaded LJP/SC/GE microcapsules reached about 93.4%, and the survival rate was 46.9% in simulated gastric fluid (SGF) for 2 h and 96.0% in simulated intestinal fluid (SIF) for 2 h. In vitro release experiments showed that the LJP/SC/GE microcapsules could protect the viability of L. plantarum in SGF and release probiotics slowly in SIF. The cell survival of LJP/SC/GE microcapsules was significantly improved during the heat treatment compared to SC/GE microcapsules and free cells. LJP/SC/GE microcapsules can increase the survival of L. plantarum by maintaining the lactate dehydrogenase and Na+-K+-ATPase activity. Overall, this study demonstrates the great potential of LJP/SC/GE microcapsules to protect and deliver probiotics in food and pharmaceutical systems.

Funder

Natural Science Foundation of Xiamen

Science and Technology Program of Fujian Province

Xiamen Southern Oceanographic Center

Publisher

MDPI AG

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