Interpreting Seasonal Patterns and Long-Term Changes of Zooplankton in a Deep Subalpine Lake Using Stable Isotope Analysis

Abstract

The purpose of this study was to elucidate the patterns and mechanisms driving seasonal and interannual variations of carbon and nitrogen stable isotopes in the zooplankton crustacean community of Lake Maggiore (Italy), during the period 2009–2020. Different zooplankton taxa and groups showed different ranges of δ13C signatures, giving an insight into food sources and niche partition. In particular, cyclopoids had a restricted range with more negative δ13C‰ values and an increase in δ13C fractionation with the establishment of water thermal vertical stratification, highlighting the importance of vertical distribution as a key factor for taxa coexistence in a vertically heterogenous environment. The δ13C values of the zooplankton community and of Daphnia were positively related to water temperature (R2 = 0.58 p < 0.0001 and R2 = 0.68 p < 0.0001, respectively), and the δ13C Daphnia signature was positively related to chlorophyll a (R2 = 0.32, p < 0.0001). Decomposition of the time-series data for zooplankton carbon and nitrogen signatures and environmental parameters identified increasing trends in water temperature, chlorophyll a and water conductivity and a decrease in nitrate that matched changes in carbon isotopic signature trends in some zooplankton taxa (Bosmina, Daphnia and Diaptomids). Overall, the observed patterns in zooplankton isotopic signatures were interpreted as integrations of the effects of climate warming in Lake Maggiore, affecting both the availability of food sources and environmental conditions.