Affiliation:
1. Agronomy College, Guizhou University, Huaxi, Guiyang 550025, China
Abstract
The regulation of microsomal (e.g., FAD2) and plastidial (e.g., FAD6) oleate desaturases by cold, heat and salt stress were investigated. Gene expression levels and fatty acid compositions were determined in the roots, stems and leaves of safflower following stress treatments. A safflower plastidial oleate desaturase gene, CtFAD6, was cloned, and oleic acid desaturation was confirmed in Synechococcus sp. strain PCC7942. The results showed that temperature regulated oleate desaturation at the transcriptional level, and this regulation pattern was tissue-specific. CtFAD2-1, CtFAD2-2 and CtFAD6 were significantly induced under cold and heat stress in young leaves, and CtFAD2-2 and CtFAD6 were slightly induced in young stems. In contrast, CtFAD2-1, CtFAD2-11 and CtFAD2-10 were sensitive to salt stress in all safflower tissues (roots, stem and leaves). CtFAD6 was insensitive to salt and was slightly induced in leaves only.
Funder
Guizhou Province Science and Technology Project
Talent Introduction Project of Guizhou University
Guiyang City Science and Technology Project
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference32 articles.
1. Lipid modulation of plasma membrane-bound ATPase;Cooke;Physiol. Plantarum.,2010
2. Cao, S.J., Zhou, X.R., Wood, C.C., Green, A.G., Singh, S.P., Liu, L., and Liu, Q. (2013). A large and functionally diverse family of FAD2 genes in safflower (Carthamus tinctorius L.). BMC Plant Biol., 13.
3. The FAD2 Gene in Plants: Occurrence, Regulation, and Role;Dar;Front. Plant Sci.,2017
4. Lipid modulation of transport proteins in vertebrate cell membranes;Deuticke;Annu. Rev. Physiol.,1987
5. Guan, L.L. (2011). Accumulation pattern and regulatory mechanisms of fatty acid in different safflower (Carthamus tinctorius L.) tissues. [Dissertation Thesis, Sichuan Agriculture University].
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献